![]() Make sure you are clear on what constitutes a dilution factor. When you do serial dilutions, you multiply together all of the dilution factors. Pull out 1 mL of this latest mixture, and you'll have 1/100th of 1/100th, which is (multiplying the fractions together) 1/10,000th.Now you can take 1 mL of THAT solution, add it to a new container, top off with 99mLs of water, and you'll have only 1/100th of the original bacteria in the mL.You still have the same number of bacteria, but now they're spread out. Take that 1 mL and put it in 99 mLs of saline solution.That would be pretty difficult with a pipette. ![]() Why would you want to do this? Let's say you need to get 1/10,000th of a mL in order to count the bacteria in it. Frequently, you will find it necessary to add water (or some other medium) to a stock (or soup, get it?) with a known concentration to make a more dilute solution. The story on the previous pages has many parallels with life in a microbiology lab.
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